Stool or enema specimens from California inpatients or outpatients with suspected IB may be submitted directly to the IBTPP laboratory. Physicians seeking testing for their patients should first call the IBTPP on-call physician at (510) 231-7600 prior to specimen submission to provide case information and review indications for testing.
Testing will not be initiated without prior clinical consultation with the IBTPP on-call physician.
The specimen required for the definitive diagnosis of infant botulism is stool or enema. The best container in which to collect, store and submit fecal specimens is a sterile urine container with a tight, screw-capped lid. Do not use containers containing fixatives such as those used for ovum and parasite collection. If spontaneously passed stool is difficult to obtain due to constipation, an attempt to collect stool in the rectal vault should be made by gentle digital examination. If no stool can be obtained digitally, do not wait for a spontaneous bowel movement. Instead, please follow the enema collection procedure outlined below.
Important: Glycerin suppositories yield an unsatisfactory specimen and should not be used. The procedure described below will yield the best enema specimen for diagnostic testing.
Enema Collection Procedure
Please retain all subsequent stool specimens and have your laboratory store them in their refrigerator until a diagnosis has been established by the appropriate laboratory. Please do not send extra stool specimens unless requested. For California only - please contact 510-231-7676 with any questions regarding specimen collection, storage or submission requirements. For all other states, please contact your state health department. Thank you.